Promoting scopolamine accumulation in transgenic plants of Atropa belladonna generated from hairy roots with over expression of pmt and h6h gene

نویسندگان

  • Xiaoqiang Liu
  • Chunxian Yang
  • Min Chen
  • Mingyang Li
  • Zhihua Liao
  • Kexuan Tang
چکیده

Laboratory of Natural Products and Metabolic Engineering, Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, People’s Republic of China. School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, People’s Republic of China. Institute of Ornamental Plants Research, Southwest University, Chongqing 400715, People’s Republic of China. Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, Shanghai Jiao Tong University, Shanghai 200030, People’s Republic of China.

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منابع مشابه

Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes

Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...

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Enhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L.

Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...

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Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase.

Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter and integrated into the Atropa belladonna genome. Transgenic plants and derived root cultures were analysed for gene expression and for levels of alkaloids and their precursors. Scopolamine, hyoscyamine, tr...

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Differential production of tropane alkaloids in hairy roots and in vitro cultured two accessions of Atropa belladonna L. under nitrate treatments.

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane...

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Metabolic engineering of plant alkaloid biosynthesis.

Plant alkaloids, one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberine have been cloned, making the metabolic engineering of these alkaloids possible. Expression of two branching-point enzymes was engineered: putrescine N-methyltransferase (PMT) in transgenic plants of A...

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تاریخ انتشار 2010